Published date: 2 October 2019

Last edited date: 2 October 2019

This notice was replaced on 16 January 2020

This notice does not contain the most up-to-date information about this procurement. The most recent notice is:

Awarded contract (published 16 January 2020)

Closed opportunity - This means that the contract is currently closed. The buying department may be considering suppliers that have already applied, or no suitable offers were made.


Contract summary

Industry

  • Medical analysis services - 85148000

Location of contract

North East

Value of contract

£40,000 to £100,000

Procurement reference

tender_219709/800346

Published date

2 October 2019

Closing date

9 October 2019

Contract start date

14 October 2019

Contract end date

13 October 2021

Contract type

Service contract

Procedure type

Open procedure (below threshold)

Any interested supplier may submit a tender in response to an opportunity notice.

This procedure can be used for procurements below the relevant contract value threshold.

Contract is suitable for SMEs?

Yes

Contract is suitable for VCSEs?

No

Description

Public Health England (PHE) is looking for a whole genome sequencing (WGS) provider to perform enrichment and sequencing of meningococcal DNA extracted directly from clinical specimens.
PHE's Meningococcal Reference Unit (MRU) carries out enhanced surveillance of invasive meningococcal disease (IMD) in England and Wales (E&W). This is of particular importance following the introduction of the novel, protein-based 4CMenB (Bexsero®) meningococcal vaccine into the national infant immunisation schedule in September 2015. Data from a recent study pilot study indicates that meningococcal RNA baits can be used to target and isolate meningococcal DNA from within a mixed (human and bacterial) extracts (Clark et al. 2017). As part of PHE Enhanced Surveillance activities, three years of clinical sample extracts (n=100-170, depending on available sample) are to be whole genome sequenced using this approach.
The requirements include:
- Targeted-enriched sequencing library preparation:
- Hybridization and Capture of target DNA using Neisseria meningitidis-specific RNA baits
- Post-capture amplification
- QC and sample pooling
- Library Sequencing
- Bioinformatics:
- Screening of human reads
- Mapping
- De novo assembly

Reference
Clark SA, Doyle R, Lucidarme J, Borrow R, Breuer J. Targeted DNA enrichment and whole genome sequencing of Neisseria meningitidis directly from clinical specimens. Int J Med Microbiol 2017; 308: 256-262.

If you are interested in participating in this opportunity please login/register with PHE's sourcing platform Bravo https://phe.bravosolution.co.uk to access to the tender documents.

More information

Links

Additional text

If you are interested in participating in this opportunity please login/register with PHE's sourcing platform Bravo.

About the buyer

Address

133-155 Waterloo Road
London
SE1 8UG
England

Email

anna.gibson@phe.gov.uk