Published date: 30 March 2022

Last edited date: 30 March 2022

Closed opportunity - This means that the contract is currently closed. The buying department may be considering suppliers that have already applied, or no suitable offers were made.


Closing: 12 April 2022, 12pm

Contract summary

Industry

  • Analysis services - 71620000

  • Environmental services - 90700000

Location of contract

Any region

Value of contract

£30,000

Procurement reference

tender_303038/1056958

Published date

30 March 2022

Closing date

12 April 2022

Closing time

12pm

Contract start date

19 April 2022

Contract end date

28 June 2022

Contract type

Service contract

Procedure type

Open procedure (below threshold)

Any interested supplier may submit a tender in response to an opportunity notice.

This procedure can be used for procurements below the relevant contract value threshold.

Contract is suitable for SMEs?

Yes

Contract is suitable for VCSEs?

No

Description

Cefas require the successful supplier to provide services for DNA to be extracted from 116 frozen Sterivex cartridges (Millipore; SVGP01050). The cartridges consist of 29 samples in triplicate, and 29 field blanks. Following extraction, Cefas require half the extracted DNA to be sent back to the Cefas laboratory in Lowestoft so we can undertake qPCR (e.g., each of the extracted samples will be halved, one half will be used for metabarcoding, while the other half will be used for qPCR).

Cefas require the extracted DNA to undergo metabarcoding using the MiFish 12s marker (Miya et al., 2015) (and MiFish T markers if dual amplicon approach can be achieved). The supplier will also conduct taxonomic assignment on the metabarcoding data using amplicon sequence variants (preferred) or operational taxonomic units and Blast on a Genbank generated database (or similar). Metabarcoding results should be presented to Cefas so that the number of sequences per taxa is available for every sample in a table format.

Cefas also require the supplier to provide the raw data for the sequencing run and methodology involved. Cefas will report results in a scientific manuscript, in consequence details on the methodology provided by the supplier will need to be sufficient to pass peer review (proprietary techniques will be protected).

Cefas require the supplier to provide weekly updates via email detailing progress on deliverables.

Cefas require all deliverables to be completed within 10 weeks of Contract start date.

More information

Links

About the buyer

Address

Cefas Lowestoft Laboratory
Lowestoft
NR33 0HT
England

Email

procure@cefas.co.uk