Published date: 13 May 2024

Closed opportunity - This means that the contract is currently closed. The buying department may be considering suppliers that have already applied, or no suitable offers were made.


Closing: 13 June 2024, 12pm

Contract summary

Industry

  • Research services - 73110000

Location of contract

London

Value of contract

£167,000

Procurement reference

BIP861438760

Published date

13 May 2024

Closing date

13 June 2024

Closing time

12pm

Contract start date

29 July 2024

Contract end date

29 July 2025

Contract type

Service contract

Procedure type

Open procedure

Any interested supplier may submit a tender in response to an opportunity notice.

Contract is suitable for SMEs?

Yes

Contract is suitable for VCSEs?

No

Description

Royal Botanic Gardens, Kew wishes to appoint a supplier to individually sequence the whole genomes of up to 300 European ash trees (Fraxinus excelsior) at a minimum of 15x whole genome coverage via a long-read technology. Fraxinus excelsior is a diploid and has a 1C genome size of 880 Mbp.

In addition to the 300 individual ash trees, we wish to sequence a minimum of six technical replicates. Therefore, the total number of samples we wish to sequence is 306.

RBG Kew intends to supply total genomic DNA for each sample with the following metrics: minimum of 10ug DNA; minimum concentration of 30ng/ml (as measured with a Quantus flurometer dsDNA assay); at least 50% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1.8; minimum OD260/230 of 1.8; minimum DIN of 7.

More information

Links

About the buyer

Address

The Herbarium, Kew Green
Richmond,
London
TW9 3AE
UK

Telephone

+442083325000

Email

procurement@kew.org