Published date: 20 August 2024

Last edited date: 20 August 2024

This notice was replaced on 20 August 2024

This notice does not contain the most up-to-date information about this procurement. The most recent notice is:

Opportunity (published 20 August 2024, last edited 20 August 2024)

Closed opportunity - This means that the contract is currently closed. The buying department may be considering suppliers that have already applied, or no suitable offers were made.


Contract summary

Industry

  • Analysis services - 71620000

  • Research laboratory services - 73111000

Location of contract

East of England

Value of contract

£50,000 to £80,000

Procurement reference

CF-0351400D8d000003VQwdEAG

Published date

20 August 2024

Closing date

10 September 2024

Closing time

12pm

Contract start date

30 September 2024

Contract end date

15 March 2025

Contract type

Service contract

Procedure type

Open procedure (below threshold)

Any interested supplier may submit a tender in response to an opportunity notice.

This procedure can be used for procurements below the relevant contract value threshold.

Contract is suitable for SMEs?

Yes

Contract is suitable for VCSEs?

No


Description

Cefas is currently undertaking genetics based Close-Kin Mark-Recapture (CKMR) research on pollack populations. Cefas is seeking a supplier to sequence samples using a Genotype-by-Sequencing (GBS) approach. Samples have been collected from pollack originating from multiple areas across the English Channel with generated Single Nucleotide Polymorphisms (SNPs) being used to identify Parent-Offspring Pairs (POPs) as well as more distant kinship relationships, such as Half Sibling Pairs (HSPs).

Cefas is aware that there is a multitude of GBS approaches, some using propriety technology and some not. Cefas are seeking applications which offer a GBS approach that maximises SNP identification and coverage, to allow Cefas to explore kinship levels amongst pollack.

To aid with the above project, Cefas require the supplier to carry out DNA extraction of muscle tissues, quality control steps (DNA Quality Control information (i.e. pass/fail based on DNA concentration and DNA quality) supplied to Cefas before genotype-by-sequencing commences) and genotype-by-sequencing of pollack samples. Therefore, suppliers are asked to provide a cost per sample based on the above.

As standard, muscle tissue samples are to be provided by Cefas in LVL genetic sampling tubes with analytical grade ethanol and stored in -20oC:
https://www.lvl-technologies.com/en/sample-storage/safe-2d-tubes-engl/safe-96-tissue-collection-951.

Cefas requires suppliers to provide an estimate of how long it would take to provide results for both 3000 samples and their individual minimum run length to process per sequencing, based on the 'Deliverables' section described below.

Deliverables:
• DNA extraction of all samples
• DNA Quality Control information (i.e. pass/fail based on DNA concentration and DNA quality) supplied to Cefas before genotype-by-sequencing commences.
• Genotype-by-sequencing providing SNPS with an average of >30x coverage.
o ~3000* tissue samples provided in total (exact amount to be confirmed)
• Samples can either be submitted to the supplier as they are received by Cefas OR submitted all at once based on suppliers' flexibility
• Sequencing files to be provided in fastq format with a QC Report
o Final sample submission date being end of February 2025.
o All results, with a final QC report, must be received by 15th of March 2025.

*Please note sample number stated above is the current estimated number of samples collected, final sample number may vary

Location for services to be undertaken: At the supplier's premises - all steps of the process must take place within the United Kingdom to ensure deterioration of samples

Download the Bidder Pack at https://defra-family.force.com/s/Welcome and search for CEFAS24-116 under opportunities.


More information

Previous notice about this procurement

CEFAS24-116 Request for Quotation for genotype-by-sequencing approach for estimating familial relations among pollack

  • Opportunity
  • Published 20 August 2024


About the buyer

Contact name

Jade Peek

Address

Pakefield Road
Lowestoft
NR33 0HT
GB

Email

procure@cefas.gov.uk