Published date: 21 June 2022
This notice was replaced on 25 August 2022
This notice does not contain the most up-to-date information about this procurement. The most recent notice is:
Awarded contract (published 25 August 2022)
Closed opportunity - This means that the contract is currently closed. The buying department may be considering suppliers that have already applied, or no suitable offers were made.
Contract summary
Industry
Laboratory services - 71900000
Research laboratory services - 73111000
Location of contract
TW9 3AE
Value of contract
£57,000
Procurement reference
RBGKEW1066 Long-Read Ash Genome Sequencing
Published date
21 June 2022
Closing date
19 July 2022
Closing time
12pm
Contract start date
8 August 2022
Contract end date
8 February 2023
Contract type
Service contract
Procedure type
Open procedure (below threshold)
Any interested supplier may submit a tender in response to an opportunity notice.
This procedure can be used for procurements below the relevant contract value threshold.
Contract is suitable for SMEs?
Yes
Contract is suitable for VCSEs?
No
Description
RBG Kew wishes to appoint a supplier to individually sequence the whole genomes of up to 50 European ash trees (Fraxinus excelsior) at a minimum of 15x whole genome coverage via a long-read technology. Fraxinus excelsior has a 1C genome size of 880 Mbp.
RBG Kew intends to supply total genomic DNA for each tree with the following metrics: minimum of 20ug DNA; minimum concentration of 30ng/ul (as measured with a Quantus flurometer dsDNA assay); at least 80% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1.7; minimum OD260/230 of 1.7.
Based on a fixed budget of £57,000 GBP (excluding VAT), we wish to maximise: firstly, the number of trees sequenced at a minimum of 15x whole genome coverage (minimum of 13.2 gigabases of data per sample); secondly, in the event that all 50 trees can be sequenced to at least 15x (minimum of 13.2 gigabases of data per sample), the total amount of data in gigabases for each of the 50 trees.
You are hereby invited by the Board of Trustees of the Royal Botanic Gardens, Kew to submit a tender to meet the Customer's requirements in accordance with the specification and associated documents as contained within this tender pack.
Tender documents can be accessed via:
https://defra.bravosolution.co.uk
https://defra.bravosolution.co.uk/go/08518853018185EF98F9
itt_10303
project_36622
Title:- RBGKEW1066 Long-Read Ash Genome Sequencing 2022
Suppliers will be required to register with BRAVO, if not already registered. All tender documents can only be accessed via BRAVO
More information
Additional text
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Tender documents can be accessed via:
https://defra.bravosolution.co.uk
https://defra.bravosolution.co.uk/go/08518853018185EF98F9
itt_10303
project_36622
Title: RBGKEW1066 Long-Read Ash Genome Sequencing 2022
Suppliers will be required to register with BRAVO if not already registered. All tender documents can only be accessed via BRAVO
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