Published date: 20 April 2022

Last edited date: 20 April 2022

Awarded contract - This means that the contract has been awarded to a supplier.


Closing: 12 April 2022, 12pm

Contract summary

Industry

  • Analysis services - 71620000

Location of contract

Any region

Value of contract

£20,880

Procurement reference

tender_303038/1065114

Published date

20 April 2022

Closing date

12 April 2022

Closing time

12pm

Contract start date

20 April 2022

Contract end date

30 June 2022

Contract type

Service contract

Procedure type

Open procedure (below threshold)

Any interested supplier may submit a tender in response to an opportunity notice.

This procedure can be used for procurements below the relevant contract value threshold.

Contract is suitable for SMEs?

Yes

Contract is suitable for VCSEs?

No

Description

Cefas has contracted the supplier to provide services for DNA to be extracted from 116 frozen Sterivex cartridges (Millipore; SVGP01050). The cartridges consist of 29 samples in triplicate, and 29 field blanks. Following extraction, Cefas require half the extracted DNA to be sent back to the Cefas laboratory in Lowestoft so we can undertake qPCR (e.g., each of the extracted samples will be halved, one half will be used for metabarcoding, while the other half will be used for qPCR).

Cefas require the extracted DNA to undergo metabarcoding using the MiFish 12s marker (Miya et al., 2015) (and MiFish T markers if dual amplicon approach can be achieved). The supplier will also conduct taxonomic assignment on the metabarcoding data using amplicon sequence variants (preferred) or operational taxonomic units and Blast on a Genbank generated database (or similar). Metabarcoding results should be presented to Cefas so that the number of sequences per taxa is available for every sample in a table format.

The supplier will also provide the raw data for the sequencing run and methodology involved. Cefas will report results in a scientific manuscript, in consequence details on the methodology provided by the supplier will need to be sufficient to pass peer review (proprietary techniques will be protected).

The supplier will provide weekly updates via email detailing progress on deliverables.

The supplier will provide all deliverables within 10 weeks of Contract start date.

More information

Attachments

Award information

Awarded date

20 April 2022

Contract start date

20 April 2022

Contract end date

30 June 2022

Total value of contract

£20,880

This contract was awarded to 1 supplier.

Applied Genomics Ltd.

Address

Asteroceras Science Park, Unit A, Greengates Way, Hoveton, Norfolk NR12 8ED

Reference

No reference - other

Supplier is SME?

No

Supplier is VCSE?

No

About the buyer

Address

Cefas Lowestoft Laboratory
Lowestoft
NR33 0HT
England

Email

procure@cefas.co.uk