Published date: 31 January 2017

Closed opportunity - This means that the contract is currently closed. The buying department may be considering suppliers that have already applied, or no suitable offers were made.


Closing: 1 March 2017

Contract summary

Industry

  • Detection and analysis apparatus - 38430000

  • Analysis apparatus - 38432000

  • Spectrometers - 38433000

    • Analysers - 38434000

Location of contract

LS2 9JT

Value of contract

£0

Procurement reference

JLB/F2627

Published date

31 January 2017

Closing date

1 March 2017

Contract start date

31 March 2017

Contract end date

31 March 2018

Contract type

Supply contract

Procedure type

Open procedure

Any interested supplier may submit a tender in response to an opportunity notice.

Contract is suitable for SMEs?

No

Contract is suitable for VCSEs?

No

Description

The FFF/SEC-multidetection instrument is for separating, characterising, and fractionating both biomacromolecules and biological particles. The biomacromolecules include biopolymers such as polysaccharides (e.g., glycosaminoglycans), proteins, nucleic acids, mucins, while the biological particles include antibodies, nanocomplexes, exosomes, mucus, among other. These systems are to be analysed as pure or as mixtures. The separation is achieved either by size exclusion HPLC (SEC-HPLC) or by asymmetrical flow field-flow fractionation (AsFlFFF) under thermostated conditions. In both cases, this is according to the dimensions given by the molar mass and hydrodynamic size of the polymers and particles. In both separation modes, the system enables the determination of the molar mass, hydrodynamic radius (Rh), radius of gyration (Rg,) polymer conformation and branching degree of biopolymers, as well as the particle size (Rh) of nano- and micro-particles. The characterisation is made by a multidetection system that uses dynamic and multi-angle laser light scattering (DLS and MALS, respectively), while the composition is elucidated by a UV/VIS spectroscopy and a differential refractive index (DRI) detectors. The fractionated eluting polymer and particle species pass through all these detector units arranged online, and the software integrates the information to generate the corresponding chromatograms (SEC) and fractograms (FFF) as well as the molecular mass, Rg, and particle size (Rh) distribution plots and parameters derived thereof. The separated fractions can subsequently be recovered by an automated fraction collector system.

More information

Links

Additional text

Is a Recurrent Procurement Type? : No

About the buyer

Contact name

Jo Burns

Address

University of Leeds
Leeds
West Yorkshire
LS2 9JT
United Kingdom

Telephone

0113 3433467

Email

j.l.burns@leeds.ac.uk